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人胰岛素样生长因子-1的体外抗肝纤维化活性
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引用本文:万爱妮,徐栋生,蔡燕飞,陈蕴,金坚,李华钟.人胰岛素样生长因子-1的体外抗肝纤维化活性[J].中国药科大学学报(中文版),2017,48(4):476-482
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作者单位
万爱妮 江南大学生物工程学院 
徐栋生 药学院 
蔡燕飞 药学院 
陈蕴 药学院 
金坚 药学院 
李华钟 江南大学生物工程学院 
基金项目:国家高技术研究发展计划(863计划)资助项目(No.2014AA021003);江苏省优势学科建设工程资助项目(PADA)
中文摘要:探讨人胰岛素样生长因子-1(IGF-1)的体外抗肝纤维化作用。检测IGF-1对人正常肝细胞L-02增殖及生长周期的影响;建立CCl4诱导的肝细胞损伤模型,探究IGF-1抗肝细胞损伤活性;以TGF-β1诱导的肝星状细胞HSC-T6为体外肝纤维化研究模型,检测IGF-1对HSC-T6细胞中纤维化相关蛋白表达水平及对胞内TGF-β1/Smad信号通路的影响。结果显示,IGF-1可解除TGF-β1对L-02生长的抑制作用,提高CCl4损伤L-02的细胞存活率,降低HSC-T6细胞纤维化相关蛋白表达水平,抑制TGF-β1/Smad信号通路中Smad3的磷酸化。结果表明,IGF-1能够促进肝细胞增殖,保护肝细胞免受损伤,干预TGF-β1/Smad信号通路,抑制胞外基质ECM合成,从而发挥抗肝纤维化效果。
中文关键词:胰岛素样生长因子-1  转化生长因子-β1  肝细胞  肝星状细胞  肝纤维化
 
Anti-liver fibrosis activities of human insulin-like growth factor-1 in vitro
Abstract:This study was focus on investigating the anti-liver fibrosis effects of insulin-like growth factor-1(IGF-1) in vitro. The effects of IGF-1 on human liver L-02 cell viability and cell cycle were observed. CCl4-induced L-02 cell injury was set up to detect the anti-apoptotic activity of insulin-like growth factor-1(IGF-1). Transforming growth factor β1(TGF-β1)induced hepatic stellate cell line(HSC-T6)were used as a liver fibrosis model in vitro to analyze the effects of IGF-1 on the expression of liver fibrosis proteins and intracellular TGF-β1/Smad signaling pathway in HSC-T6 cells. The results showed that IGF-1 could relieve the growth inhibition effects of TGF-β1 on L-02 cells, increase the viability of L-02 cells injured by CCl4, decrease the expression of liver fibrosis proteins, and inhibit the TGF-β1/Smad signaling pathway by inhibiting the phosphorylation of Smad3. Our study suggested that IGF-1 exerted anti-liver fibrosis effects by stimulating L-02 cells proliferation, reducing cell damage and inhibiting ECM accumulation via interfering TGF-β1/Smad signaling pathway.
keywords:insulin-like growth factor-1  transforming growth factor β1  liver cells  hepatic stellate cells  liver fibrosis
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