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酶-二氧化锰纳米片杂化探针自指示比色传感葡萄糖
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引用本文:李莉,许风国,陈金龙.酶-二氧化锰纳米片杂化探针自指示比色传感葡萄糖[J].中国药科大学学报(中文版),2017,48(4):453-460
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作者单位
李莉 中国药科大学药物分析学教研室 
许风国 中国药科大学药物分析学教研室中国药科大学药物质量与安全预警教育部重点实验室 
陈金龙 中国药科大学药物分析学教研室中国药科大学药物质量与安全预警教育部重点实验室南开大学药物化学生物学国家重点实验室 
基金项目:国家自然科学基金资助项目(No.21305161);江苏省自然科学基金资助项目(No.BK20130643);药物质量与安全预警教育部重点实验室开放课题资助项目(DQCP2015MS06);药物化学生物学国家重点实验室(南开大学)开放基金资助项目(No.20130404)
中文摘要:构建一种二氧化锰纳米片与葡萄糖氧化酶杂化自指示比色探针,在弱酸性条件下,葡萄糖氧化酶专属性催化氧化葡萄糖,定量产生葡萄糖酸和过氧化氢,生成的过氧化氢有效分解二氧化锰纳米片,探针体系特征吸收波长(374 nm)处吸收度降低。吸收度变化量与葡萄糖浓度呈线性相关,线性范围1~20 μmol/L,相关系数R2=0.990 1,检测限低至0.1 μmol/L。探针对葡萄糖具有高选择性和灵敏度,血清样品中常见的氨基酸、阴离子、阳离子及蛋白质等小分子物质造成干扰均可忽略,实际样品测定具有前处理简单、操作方便、快速等特点。此外,鉴于二氧化锰的氧化还原特性以及酶催化体系的多样性,酶-二氧化锰纳米片杂化探针平台具有普适性,可设计构建多种分析物的比色传感器。
中文关键词:葡萄糖  传感器  比色法  二氧化锰  纳米片  探针  葡萄糖氧化酶
 
Exploration of enzyme-MnO2 nanosheets hybridization probe for sensitively colorimetric self-indicating of glucose
Abstract:A colorimetric self-indicating probe for glucose was constructed by self-assembly of MnO2 nanosheets(MnO2 NSs)and glucose oxidase(GOD)in this paper. Under the weak acidic medium, glucose oxidase specifically catalyzes glucose into gluconic acid and hydrogen peroxide. The by-product of hydrogen peroxide could efficiently dissolve the MnO2 nanosheets, resulting into a significant decrease of the characteristic absorbance at 374 nm assigned to MnO2 NSs. Furthermore, the absorbance difference was linearly proportional to the concentration of glucose ranging from 1 to 20 μmol/L. The fitted curve could be used for quantification of glucose with a correlation coefficient of 0. 990 1. And the detection limit as low as 0. 1 μmol/L could be reached based on the definition of three times of the deviation of the blank signal(3σ)and there was negligible interference with other co-existing amino acids, anions, cations and protein, which indicated high sensitivity and selectivity of the hybrid probe. The construction strategy of designated probe is readily generalized in principle for detection of numerous analytes in view of reactive property of MnO2 and the diversity of enzymes.
keywords:glucose  sensor  colorimetry  MnO2  nanosheets  probe  glucose oxidase
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