国家自然科学基金资助项目(No. 81930099, 81773664, 81473153)
Center of Advanced Pharmaceuticals and Biomaterials, China Pharmaceutical University, Nanjing 210009, China
脂筏（Lipid raft）是由饱和磷脂、鞘磷脂以及胆固醇组成的位于细胞膜的液态有序微区，与细胞的许多生理/病理过程密切相关。基于脂筏区与非脂筏区脂质组成与分布的差异，本文设计并合成了一种具有聚集诱导发光（Aggregation-induced emission，AIE）特性的脂筏探针胆固醇-三聚乙二醇-四苯乙烯（TCHS-TPE）用于细胞膜上脂筏区的便捷特异性成像。本文首先成功合成了TCHS-TPE，同时测定了TCHS-TPE的光物理性质以评价其AIE特性，最后采用激光共聚焦显微镜研究了TCHS-TPE对B16F10黑色素瘤细胞膜上脂筏区的特异性成像。与现有脂筏探针霍乱毒素B相比，TCHS-TPE脂筏探针具有操作简单，特异性高等优势。该荧光探针的成功合成将为研究脂筏区相关生理、病理过程提供有利工具，并为其他脂筏区成像探针的设计提供理论基础。
raft domains are usually defined as tiny, highly dynamic and transient plasma membrane regions that are composed of saturated phospholipids, sphingolipids, glycolipids and cholesterol, which are involved in many physiological and pathological processes. Based on the difference in composition and physical properties between lipid raft and non-raft domains, Cholesterol-triethylene glycol-tetraphenylethylene (TCHS-TPE) with Aggregation-induced emission (AIE) for convenient and specific visualizing lipid raft was designed and synthesized. We first measured the photophysical properties of TCHS-TPE to evaluate its AIE characteristics, and finally used laser confocal microscopy to study the specificity of TCHS-TPE for B16F10 melanoma cell membrane lipid raft imaging. Compared with the existing lipid raft imaging reagent cholera toxin B, the TCHS-TPE lipid raft probe has the advantages of simple operation and high specificity. The successful synthesis of the fluorescent probe will provide a useful tool for studying the physiological and pathological processes related to the lipid raft, and provide a theoretical basis for the design of imaging probes for other lipid raft regions.