一种新型锰金属有机框架纳米载体用于克服肿瘤缺氧增效光动力治疗
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国家重点基础研究发展计划资助项目(No.2017YFA0205400);国家自然科学基金资助项目(No.81603043,No.81473146)


Novel nano-scaled Mn-metal-organic framework for enhancing photodynamic therapy through overcoming tumor hypoxia
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    摘要:

    使用简单的溶剂法制备了一种新型的锰簇卟啉金属有机框架纳米载体(nMn-MOF),并对其体外增效光动力治疗的能力进行了考察。通过动态光散射仪、透射电镜、X射线光电子能谱对该纳米载体进行表征,采用氧探针、ICG荧光染料考察其催化过氧化氢产生氧气和光照下产生单线态氧(1O2)的能力。在细胞水平上,采用CCK-8法检测纳米载体的暗毒性和光毒性,通过荧光染色观察纳米载体改善肿瘤细胞乏氧和产生活性氧的能力。结果表明,制备的纳米级的锰簇卟啉金属有机框架纳米载体具有过氧化氢特异性产氧和产生高水平单线态氧的能力,改善肿瘤乏氧,从而增效光动力治疗。

    Abstract:

    A Mn-clusters-porphyrin metal-organic framework nanosheet(nMn-MOF)was synthesized by coordination chelation to enhance photodynamic therapy. The nanosheet was characterized by dynamic light scattering, transmission electron microscopy and X-ray photoelectron spectroscopy. Oxygen sensor and ICG were used to investigate the production of oxygen and the singlet oxygen(1O2)generation. The cytotoxicity of the nanosheet against tumor cells were detected by CCK-8 assay, and the anti-hypoxia and oxygen-generation ability of nanosheets were investigated by fluorescence staining assay. The results indicated that this nanosheet could catalyze the intracellular H2O2 into O2, which overcame the tumor hypoxia. Furthermore, the generated oxygen was converted to cytotoxic 1O2 under the near infrared light irradiation, thereby enhancing photodynamic therapy.

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崔华媛,姚丹,汪钰翔,胡一桥.一种新型锰金属有机框架纳米载体用于克服肿瘤缺氧增效光动力治疗[J].中国药科大学学报,2019,50(6):678-685.
Cite:CUI Huayuan, YAO Dan, WANG Yuxiang, HU Yiqiao. Novel nano-scaled Mn-metal-organic framework for enhancing photodynamic therapy through overcoming tumor hypoxia[J]. J China Pharm Univ,2019,50(6):678-685.

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  • 在线发布日期: 2019-12-31
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